Autologous platelet rich plasma repair facial nerve injury

doi:10.3969/j.issn.2095-4344.2013.28.008

Abstract

Abstract:

BACKGROUND: Therapeutic methods for of peripheral facial nerve injury include surgery, physical therapy and drug treatment, but the treatment effect is not ideal in some certain cases.
OBJECTIVE: To study the effect of autologous platelet rich plasma on repair of facial nerve injury.
METHODS: The bilateral destroyed buccal nerve branches of the 10 white rabbits were put in silica gel nerve regeneration chamber, one side injected with platelet rich plasma as experimental group, the other side injected with normal saline as control group. The general observation, neuroelectrophysiology detection, histological observation, image analysis and evaluation of facial nerve regeneration recovery were performed at 8 weeks after surgery.
RESULTS AND CONCLUSION: The action potential latency of the orbicularis oris at the experimental side was significantly lower than that at the control side, and the action potential amplitude (M wave) of compound nerve muscle of the experimental side was significantly higher than that of the control side (P < 0.01). Compared with the control side, the regenerative nerves of the experimental side were more mature with more regenerative axons, and the differentiation of myelin sheath was more mature and the thickness of myelin sheath was well-distributed. Meanwhile, the diameters of axons were closed to the normal diameter, and the nerve axons were more intensive and arranged more regularly, the outer membrane of nerve fiber was thicker and the collagen fiber and elastic fiber layer were increased when compared with the control group. The number of regenerative axons of the control side was less, and the axons were distributed irregularly and poorly developed, and a large number of fibrous connective tissues were observed. The vacuolar degeneration at the control side was more than the experimental side. The regenerated nerve in the experimental side was better than the control side in the diameter of myelinated axon, area, myelin sheath thickness and axon count, and there were significant differences between two groups (P < 0.01). It indicates that platelet rich plasma has a promoting effect in the repair and regeneration of facial nerve

Key words: tissue construction, nerve tissue construction, autologous platelet rich plasma, facial nerve injury, silica gel nerve regeneration chamber, nerve growth factor, neural regeneration

CLC Number:

R318

Zhang Xing-an, Wu Shu-jiang, Lu Hai-bin, Shi Xiu-quan, Wang Hong-ling, Cao Yun-liang, Li Yuan-xiu. Autologous platelet rich plasma repair facial nerve injury[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(28): 5145-5150.

Figures/Tables 5

References

[1]Andia I, Sánchez M, Maffulli N. Platelet rich plasma therapies for sports muscle injuries: any evidence behind clinical practice? Expert Opin Biol Ther. 2011;11(4):509-518.

[2]Griffin XL, Smith CM, Costa ML. The clinical use of platelet-rich plasma in the promotion of bone healing: a systematic review. Injury. 2009;40(2):158-162.

[3]Inchingolo F, Tatullo M, Marrelli M, et al. Regenerative surgery performed with platelet-rich plasma used in sinus lift elevation before dental implant surgery: an useful aid in healing and regeneration of bone tissue. Eur Rev Med Pharmacol Sci. 2012;16(9):1222-1226.

[4]Markou N, Pepelassi E, Kotsovilis S, et al. The use of platelet-rich plasma combined with demineralized freeze-dried bone allograft in the treatment of periodontal endosseous defects: a report of two clinical cases. J Am Dent Assoc. 2010;141(8):967-978.

[5]张卫兵,洪光祥,王发斌,等.自体富血小板血浆提取液对周围神经再生的作用[J].中华实用外科杂志,2006,23(9):1143.

[6]张森林,孟昭业,董震,等.神经生长因子几丁质管修复兔面神经缺损[J].中国现代医学杂志,2003,13(3):8-10.

[7]Novikov L, Novikova L, Kellerth JO. Brain-derived neurotrophic factor promotes axonal regeneration and long-term survival of adult rat spinal motoneurons in vivo. Neuroscience. 1997;79(3):765-774.

[8]Okuda K, Kawase T, Momose M, et al. Platelet-rich plasma contains high levels of platelet-derived growth factor and transforming growth factor-beta and modulates the proliferation of periodontally related cells in vitro. J Periodontol. 2003;74(6):849-857.

[9]Hernández-Cortés P, Garrido J, Cámara M, et al. Failed digital nerve reconstruction by foreign body reaction to Neurolac nerve conduit. Microsurgery. 2010;30(5):414-416.

[10]Wang CY, Liu JJ, Fan CY, et al. The effect of aligned core-shell nanofibres delivering NGF on the promotion of sciatic nerve regeneration. J Biomater Sci Polym Ed. 2012; 23(1-4):167-184.

[11]Serpe CJ, Byram SC, Sanders VM, et al. Brain-derived neurotrophic factor supports facial motoneuron survival after facial nerve transection in immunodeficient mice. Brain Behav Immun. 2005;19(2):173-180.

[12]Guntinas-Lichius O, Irintchev A, Streppel M, et al. Factors limiting motor recovery after facial nerve transection in the rat: combined structural and functional analyses. Eur J Neurosci. 2005;21(2):391-402.

[13]Fansa H, Keilhoff G, Förster G, et al. Acellular muscle with Schwann-cell implantation: an alternative biologic nerve conduit. J Reconstr Microsurg. 1999;15(7):531-537.

[14]李少兵,韩卉.神经营养因子与周围神经再生[J].解剖科学进展. 2001,7(3):265-270.

[15]王栓科,张致英,洪光祥.周围神经损伤与轴突诱向因子[J].现代康复,2001,5(18):54-55.

[16]陈炜,陈继川,姬长友,等. 局部应用神经营养因子对面神经损伤修复作用的试验研究[J].重庆医学,2007,36(3):207-208.

[17]刘亚峰,高炳庆,梁传余,等.神经生长因子在面神经损伤修复中作用的实验研究[J].中华耳鼻咽喉科杂志,1998,33(1):27-29.

[18]张治军,高炳庆,梁传余,等. 胰岛素样生长因子对面神经损伤的修复作用[J].中国中西医结合耳鼻咽喉科杂志,2004,12(1):7-9.

[19]Sánchez AR, Sheridan PJ, Kupp LI. Is platelet-rich plasma the perfect enhancement factor? A current review. Int J Oral Maxillofac Implants. 2003;18(1):93-103.

[20]仇建军,张长青,袁霆,等.富血小板血浆及所含相关因子在组织修复再生中的应用与作用[J].中国组织工程研究与临床康复,2009, 13(41):8131-8134.

[21]Yol S, Tekin A, Yilmaz H, et al. Effects of platelet rich plasma on colonic anastomosis. J Surg Res. 2008;146(2):190-194.

[22]Kajikawa Y, Morihara T, Sakamoto H, et al. Platelet-rich plasma enhances the initial mobilization of circulation-derived cells for tendon healing. J Cell Physiol. 2008;215(3):837-845.

[23]Landesberg R, Moses M, Karpatkin M. Risks of using platelet rich plasma gel. J Oral Maxillofac Surg. 1998;56(9): 1116- 1117.

[24]Robiony M, Polini F, Costa F, et al. Osteogenesis distraction and platelet-rich plasma for bone restoration of the severely atrophic mandible: preliminary results. J Oral Maxillofac Surg. 2002;60(6):630-635.

[25]Landesberg R, Roy M, Glickman RS. Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation. J Oral Maxillofac Surg. 2000;58(3):297-301.

[26]Dohan Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). Trends Biotechnol. 2009;27(3):158-167.